Jonathan the tortoise, the world’s oldest living land animal, is still chugging along at the ripe age of roughly 194. Now, scientists have discovered some of the secrets to Jonathan’s longevity by sequencing his genome for the first time, according to a study published Wednesday in Science Advances.

Jonathan, a Aldabra giant tortoise (Aldabrachelys gigantea), hatched around 1832 in the Seychelles archipelago, and he was already in his twenties when Charles Darwin published On the Origin of Species. In his fifties, Jonathan was brought to the remote island of Saint Helena, along with three other tortoises, as a gift to the British colonial governor in the 1880s. He has lived there ever since. 

Aldabra giant tortoises often become centenarians, but Jonathan is a major outlier even for his species as he approaches nearly 200 years on this planet. To better understand his extreme lifespan, researchers evaluated Jonathan’s genome based on swabs of his cheeks and compared it with the genomes of several other tortoises, including a 36-year-old named Tank.  

The results revealed that Jonathan has “unique gene variants in most aging pathways” including DNA repair, insulin regulation, mitochondrial function, and “low entropy” methylation, reports the study. 

“Aging is very complicated, and there's all these different hallmarks,” said Stephen Clark, an aging researcher at the non-profit Kallel Foundation and an author of the study, in a call with 404 Media. “That's why this Jonathan study is so interesting. We're still just at the very beginning of trying to understand aging.”

The study was not an easy lift. Clark first reached out to Jonathan’s caretakers in 2017, but it took some negotiating to figure out the best way to obtain the tortoise’s genetic information. A blood draw was deemed too risky, so Jonathan was coaxed into opening his mouth for a swab. The results were delayed by incomplete early samples, the Covid pandemic, and new regulations governing access to Jonathan. 

After years of back and forth, the samples were delivered from Saint Helena to Florida by the U.S. Space Force. The results showed that Jonathan had many gene variants the team expected to see, based on studies of other long-lived individuals and populations. These included variants associated with DNA repair, maintenance of aging-related sequences called telomeres, and the process of cellular recycling known as autophagy.

But the researchers found a surprise in Jonathan’s “methylome,” which is a set of methyl-based modifications involved in gene expression. The subset of Jonathan’s methylome related to mitochondrial processes was unusually pristine, or “low entropy.”  

“Jonathan's really the first of his kind to have this analysis, and we were also able to do the methylome for the younger tortoises,” Clark said. “We actually compared him to a five-year-old tortoise, so we had 189 years of difference between the methylomes. No one's ever done that, even in humans, because no humans live that long. That was one of the main things we were excited about: how does this methylome change with age?”

Whereas most of Jonathan’s genome showed signs of entropy commensurate with his age, his mitochondrial methylome seemed on par with that of the five-year-old tortoise, signaling that these pathways may play a special role in his advanced age. However, Clark said it would take more research to follow up on that lead, and to untangle the complicated web of genetic components that influence aging and lifespan. 

“We're interested in long-lived species like Jonathan because we can hopefully find other targets,” Clark said of his team’s work at Kallel Labs. “The ultimate goal of the company is to affect human healthspan and lifespan.”

As for Jonathan, he continues to live out his voluminous days at the governor’s residence, Plantation House, where he and his fellow tortoises have become one of the island’s tourist attractions. Saint Helena is also known for the exile of Napoleon Bonaparte, who died there a mere decade before Jonathan is thought to have been hatched. It’s amazing to think about just how much the world has changed in Jonathan’s lifetime. Long may he graze.  

Howdy, it's hump day and we're rolling along. Today we've got an update on a very strange case involving a man whose image was resurrected from the dead via AI and made to appear in front of a judge to announced "forgiveness" of his murderer. 404 Media contributor Matthew Gault had a long conversation with the man's sister, who wrote the words and generated the video, and she said that swaying the judge was the entire point.

THE BIG STORY

Her AI-Generated Video Swayed the Judge. The Court Said it Carried 'Undue Emotional Weight'

A man convicted of manslaughter in Arizona will be resentenced because an AI-generated video of his victim speaking from beyond the grave was ruled to have carried “undue emotional weight” as an impact statement. An appellate court in Arizona ruled that the manslaughter charge will remain, but the judge must reconsider the length of the man’s prison term because of AI.

In 2021, Gabriel Horcasitas shot and killed Christopher Pelkey during a road rage incident. A jury found him guilty of manslaughter. During Horcasitas’ sentencing hearing, Pelkey’s sister Stacey Wales played an AI-generated video of her brother as part of her victim’s impact statement. In the video, which she scripted with her own words that she made an AI generated likeness of Pelkey say, the AI avatar of Pelkey forgives his killer and said they “could have been friends” in another life. 

“I loved that video,” the judge said while handing down the maximum sentence of 10 and a half years.

Wales told 404 Media that her intent with the video was to sway the judge. “Isn’t that what you want human connection to be?” she said. “Do you ever come out of a movie and say: ‘Well, that was too emotional. That was too powerful. Human connection is powerful, and trying to convey that to somebody that's never known another person, this person on the earth, to convey their lifetime of 37 years down to a two-hour sentencing event is hard to do, and I think that victims and victims' families should be afforded every opportunity.”

Read the rest of this story here.

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A flock of new laws incoming. Several recently introduced pieces of legislation would, if passed, dramatically curb federal agencies’ access to Flock cameras, with the lawmakers referencing reporting by 404 Media in their explanations for why the legislation is needed. “At a time of growing concern about the unchecked power of artificial intelligence, Flock is eviscerating the very notion of privacy by installing tens of thousands of cameras in communities across America without their consent,” Bernie Sanders said.